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Program: preddiplomski
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Course number:
39781 (PDBT), 53707 (DBPI)
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Course Description
COURSE CONTENT
- Definition, nomenclature and classification of antibiotics
- Biotechnological process of antibiotic production
- Biotechnological process of tetracycline antibiotics production
- Biotechnological processes of β-lactam antibiotics production
- Biotechnological processes of aminoglycoside, macrolide and peptide antibiotics production
- Isolation and purification of antibiotics
LEARNING OUTCOMES
- select a method of antibiotic purification (extraction, ion exchange or precipitation) depending on the properties of the isolated antibiotic
- select drying method depending on antibiotic thermostability (drying by sublimation – lyophilisation, hot air drying, spray-drying, convective and contact-drying)
- select the optimal time for antibiotic biosynthesis and to explain regulatory mechanisms of the transition from trophophase to idiophase (antibiotic biosynthesis)
- select the rape pretreatment procedure regarding the physico-chemical properties of antibiotics (solubility, stability)
- select cultivation conditions (pure culture of microorganisms, process sterility, cultivation temperature, cultivation pH, agitation, aeration, foam suppression, etc.) during antibiotic biosynthesis
- determine antibiotic activity using chemical methods for quantitative determination of antibiotics
- sketch the antibiotic biosynthesis process
- compare the chemical and enzyme method of preparation of 6-aminopenicillanic acid
- compare the metabolic pathways of antibiotic biosynthesis regarding the microorganisms as producers of different antibiotics
- lead the biotechnological process of inoculum preparation and oxytetracycline production
To enrol in this course, the following courses must be completed (undergraduate studies):
- Biotechnology 1
- Microbiology
- Biochemistry 1
- Biochemistry 2
- Transport Phenomena
- Unit Operations