Antibiotic Technology

Course Coordinator

ECTS points:


Course number:
39781 (PDBT), 53707 (DBPI)

Course Description


  1. Definition, nomenclature and classification of antibiotics
  2. Biotechnological process of antibiotic production
  3. Biotechnological process of tetracycline antibiotics production
  4. Biotechnological processes of β-lactam antibiotics production
  5. Biotechnological processes of aminoglycoside, macrolide and peptide antibiotics production
  6. Isolation and purification of antibiotics


  • select a method of antibiotic purification (extraction, ion exchange or precipitation) depending on the properties of the isolated antibiotic
  • select drying method depending on antibiotic thermostability (drying by sublimation – lyophilisation, hot air drying, spray-drying, convective and contact-drying)
  • select the optimal time for antibiotic biosynthesis and to explain regulatory mechanisms of the transition from trophophase to idiophase (antibiotic biosynthesis)
  • select the rape pretreatment procedure regarding the physico-chemical properties of antibiotics (solubility, stability)
  • select cultivation conditions (pure culture of microorganisms, process sterility, cultivation temperature, cultivation pH, agitation, aeration, foam suppression, etc.) during antibiotic biosynthesis
  • determine antibiotic activity using chemical methods for quantitative determination of antibiotics
  • sketch the antibiotic biosynthesis process
  • compare the chemical and enzyme method of preparation of 6-aminopenicillanic acid
  • compare the metabolic pathways of antibiotic biosynthesis regarding the microorganisms as producers of different antibiotics
  • lead the biotechnological process of inoculum preparation and oxytetracycline production

To enrol in this course, the following courses must be completed (undergraduate studies):

  • Biotechnology 1
  • Microbiology
  • Biochemistry 1
  • Biochemistry 2
  • Transport Phenomena
  • Unit Operations
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