Antibiotic Technology

Course Coordinator

ECTS points:
4

Program:
preddiplomski

Course number:
39781 (PDBT), 53707 (DBPI)

Course Description

COURSE CONTENT

  1. Definition, nomenclature and classification of antibiotics
  2. Biotechnological process of antibiotic production
  3. Biotechnological process of tetracycline antibiotics production
  4. Biotechnological processes of β-lactam antibiotics production
  5. Biotechnological processes of aminoglycoside, macrolide and peptide antibiotics production
  6. Isolation and purification of antibiotics

LEARNING OUTCOMES

  • select a method of antibiotic purification (extraction, ion exchange or precipitation) depending on the properties of the isolated antibiotic
  • select drying method depending on antibiotic thermostability (drying by sublimation – lyophilisation, hot air drying, spray-drying, convective and contact-drying)
  • select the optimal time for antibiotic biosynthesis and to explain regulatory mechanisms of the transition from trophophase to idiophase (antibiotic biosynthesis)
  • select the rape pretreatment procedure regarding the physico-chemical properties of antibiotics (solubility, stability)
  • select cultivation conditions (pure culture of microorganisms, process sterility, cultivation temperature, cultivation pH, agitation, aeration, foam suppression, etc.) during antibiotic biosynthesis
  • determine antibiotic activity using chemical methods for quantitative determination of antibiotics
  • sketch the antibiotic biosynthesis process
  • compare the chemical and enzyme method of preparation of 6-aminopenicillanic acid
  • compare the metabolic pathways of antibiotic biosynthesis regarding the microorganisms as producers of different antibiotics
  • lead the biotechnological process of inoculum preparation and oxytetracycline production

To enrol in this course, the following courses must be completed (undergraduate studies):

  • Biotechnology 1
  • Microbiology
  • Biochemistry 1
  • Biochemistry 2
  • Transport Phenomena
  • Unit Operations