Enzyme Technology

Course Coordinator

ECTS points:
4

Program:
preddiplomski

Course number:
39780 (PDBT), 53709 (DBPI)

Course Description

COURSE CONTENT

  1. Determination of enzyme kinetic parameters for the substrate conversion to product under conditions of enzyme industrial application
  2. Biotechnological production of free enzymes
  3. Biotechnological production of immobilized enzymes
  4. Industrial application of enzymes

LEARNING OUTCOMES

  • critically evaluate the advantages and disadvantages of using membrane bioreactors in enzyme technology
  • critically evaluate the enzyme for a particular industrial application based on the kinetic parameters of the enzyme reaction
  • critically evaluate the most suitable processes for the enzyme immobilization that will be used for substrate conversion in biotechnological products
  • critically evaluate the most suitable methods of isolation and purification of extracellularly and intracellularly produced enzymes
  • explain the influence of the environmental factors and diffusion constraints on the immobilized enzyme kinetics
  • explain the influence of environmental parameters (pH, temperature and ionic strength) and substrate concentrations on the enzyme activity and enzyme stability in conditions of enzyme industrial application
  • evaluate the success of immobilization methods by comparing the enzyme activity of proteolytic and amilolytic enzymes before and after immobilization
  • perform the biosynthesis, isolation and purification of enzymes by submerged cultivation of Bacillus subtilis bacteria
  • sketch the scheme of biotechnological process of enzyme production by submerged and surface cultivation of working microorganism
  • compare advantages and disadvantages of biotechnological production of enzymes by surface cultivation of microorganism on solid substrate with respect to submerged cultivation

To enrol in this course, the following courses must be completed (undergraduate studies):

  • Biotechnology 1
  • Microbiology
  • Biochemistry 1
  • Biochemistry 2
  • Transport Phenomena
  • Unit Operations