Advancess in biotechnological processes for lactic acid production

Course Coordinator

ECTS points:


Course Description

Physiology of microorganisms (bacteria and fungi) that possess hydrolytic and fermentative activity, utilization of renewable raw materials; selection of new types of bioreactors suitable for bioprocessess with (semi-)solid substrates; screening of methods for isolation of liquid and (semi-)solid phase from heterogeneous systems; isolation and purification of produced D- and/or L-lactic acid; implementation of integrated bioprocessess; mathematical modelling.

After completion of this Module, PhD student will be able to:

  1. Integrate knowledge about different industrial bioprocesses for production of D- and/or L-lactic acid.
  2. Estimate applicability of different biocatalysts, raw materials, bioreactors and down-stream systems.
  3. Critically reason and recommend integrated bioprocess for lactic acid production as well as possibility for the integrated process embedding in biorafinery.
  4. Formulate possible solutions during determination of key bioprocess parameters, recomment type of bioprocess/biorafinery that fits to particular geo-political, socio-economic and climate area, the bioprocess for production and purification of lactic acid as well as for other highvalue biochemicals.


Lectures 16
Seminar 9
Exam: Seminar X   Oral Exam X


1. A. R. Neves, W. A. Pool, J. Kok, O. P. Kuipers, H. Santos, Overview on sugar metabolism and its control in Lactococcus lactis - The input from in vivo NMR, FEMS Microbiology Reviews 29 (2005) 531-554.
2. R. P. John, G.S. Anisha, K. M. Nampoothiri, A. Pandey, Direct lactic acid fermentation: Focus on simultaneous saccharification and lactic acid production, Biotechnology Advances 27 (2009) 145-152.
3. C. Gao, C. Ma, P. Xu, Biotechnological routes based on lactic acid production from biomass, Biotechnology Advances 29 (2011) 930-939.
4. R. Auras, B. Harte, S. Selke, An Overview of Polylactides as Packaging Materials, Macromolecular Bioscience 4 (2004) 835-864.
5. G. Reddy, Md. Altaf, B.J. Naveena, M. Venkateshwar, E.V. Kumar, Amylolytic bacterial lactic acid fermentation - A review, Biotechnology Advances 26 (2008) 22-34.
6. M.W. Bevan, M.C.R. Franssen, Investing in green and white biotech, Nature Biotechnology 24 (2006) 765-767.
7. R.P. John, K.M. Nampoothiri, A. Pandey, Fermentative production of lactic acid from biomass: an overview on process developments and future perspectives, Applied Microbiology and Biotechnology 74 (2007) 524-534.
8. N. Narayanan, P.K. Roychoudhury, A. Srivastava, L (+) lactic acid fermentation and its product polymerization, Electronic Journal of Biotechnology 7 (2004) 167-179.

Additional literature:

1. Slavica, Anita; Trontel, Antonija; Jelovac, Nuša; Kosovec, Željka; Šantek, Božidar; Novak Srđan. Production of lactate and acetate by Lactobacillus coryniformis subsp. torquens DSM 20004T in comparison with Lactobacillus amylovorus DSM 20531T. Journal of biotechnology. 202 (2015); 50-59
2. Trontel, Antonija; Batušić, Ana; Gusić, Ivana; Slavica, Anita; Šantek, Božidar; Novak, Srđan. Production of D- and L-Lactic Acid by Mono- and Mixed Cultures of Lactobacillus sp.. Food technology and biotechnology. 49 (2011), 1; 75-82.
3. Trontel, Antonija; Baršić, Vanda; Slavica, Anita; Šantek, Božidar; Novak, Srđan. Modeling the Effect of Different Substrates and Temperature on the Growth and Lactic Acid Production by Lactobacillus amylovorus DSM 20531T in Batch Process. Food technology and biotechnology. 48 (2010), 3; 352-361.