Biochemical EngIneering

Course Coordinator

ECTS points:
8

Program:
preddiplomski

Course number:
39803

Course Description

COURSE CONTENT

1. Definition, importance and range of biochemical engineering and its

    development directions

L:Definition, importance and range of biochemical engineering and its

   development directions (2 h)

2. Bioreactor systems for biotechnological production

L: Bioreactor systems for bioprocess conduction on solid, semi-solid and

    liquid substrates (6 h)

S: Calculation of design parameters for different bioreactor systems (4 h)

3. Bioprocess conduction manners – batch and fed batch process

L: Batch and fed batch processes (4 h)

S: Calculation of batch and fed batch processes (4 h)

P: Batch cultivation of bacterium Lactobacillus delbrueckii (10 h);

    Fed batch cultivation of yeast S. cerevisiae (10 h)

4. Bioprocess conduction manners – semi-continuous and continuous

    process

L: Semi-continuous and continuous processes (5 h)

S: Calculation of semi-continuous and continuous processes (4 h)

P: Semicontinuous cultivation of yeast S. cerevisiae (10 h)

5. Mixing and aeration in bioprocesses

L: Systems for mixing and aeration in different bioreactors (3 h)

S: Calculation of systems for mixinig and aeration in different bioreactors

    (4 h)

6. Monitoring, control and regulation of bioprocesses

L: Systems for monitoring, control and regulation of bioprocesses (6 h)

S: Calculation of systems for control and regulation of bioprocesses (4 h)

7. Sterilisation of equipment, media and air for biotechnological

     production

L: Batch and continuous sterilization (3 h)

S: Calculation of batch and continuous sterilization (8 h)

8. Application of mathematical models in biotechnological production

L: Formation, types and application of mathematical models in

    biotechnological production (3 h)

9. Immobilised biocatalytic systems

L: Types, preparation and application of immobilised biocatalysts (3 h)

S: Calculation of reaction kinetics and mass transfer in immobilised

    biocatalysts (2 h)

10. Separation and purification of biotechnological products

L: Separation and purification of biotechnological products (4 h)

LEARNING OUTCOMES

  • prepare cultivation media and inoculum for different bioprocess conduction manners (batch, fed batch, semi-continuous and continuous)
  • establish and solve mass balance and quantify bioprocess kinetics for different bioprocess conduction manners
  • establish and manage different bioprocess conduction manners
  • calculate bioprocess efficiency parameters for different bioprocess conduction manners
  • propose adequate equipment for bioprocess conduction on semi-solid and solid substrate as well as on liquid cultivation media
  • create and build up optimal system for monitoring and control of bioprocesses
  • calculate, establish and manage of batch and continuous sterilization of cultivation media as well as air sterilization
  • develop and use mathematical models for bioprocess control and optimization
  • propose and create systems for bioprocess conduction with immobilized biocatalysts of first and second generation
  • propose adequate process for microbial biomass separation, microbial cells disruption and separation of microbial metabolism products
  • create and build up optimal technological line for bioprocess conduction in industrial scale