Biochemical engineering and bioprocess techniques

Course Description

The course containing three methodical units:

1. Hydrodynamic characterization of bioreactors
2. High structured mathematical models of metabolic flux and bottleneck detection
3. Scale-up methods in bioprocess engineering

1) Hydrodynamic characterization of bioreactors:

Measuring of mixing time, circulation time, homogenization time, its distribution, methods for evaluation (delta function and step methods, conductivity method, pH-method, photometric methods,  radioactive tracers, radio transmitter method, temperature delta and step method, single and multi-sensors systems)

2) High structured mathematical models of metabolic flux and bottleneck detection

Structuring of mathematical models of metabolic pathways, metabolic modelling of primary metabolic products - direct related and partially related to growth, as well as the modelling of secondary metabolites synthesis). Modelling of prokaryote live-cycle, NMR and MS technics as a tool for model evaluation, sensitivity analysis and error analysis.

3) Scale-up methods in bioprocess engineering

Fundamental and semi-fundamental methods, dimensional analysis and regime analysis, rules of thumb method, try and error method.

Lectures

Lectures: 20 hours

Seminars: 10 hours

Description of course requirements:

Preparation and defense of written seminar and oral exam

Literature

1. Duran P. M. Bioprocess Engineering Principles (2^nd ed.), Academic Press, Oxford (UK), 2012
2. Marić V., Šantek B., Biokemijsko Inženjerstvo, Golden Marketing-Tehnička knjiga, Zagreb (HR), 2009
3. Vogel H.C., Todaro, L.C. Fermentation and Biochemical Engineering Handbook: Principles, Process Design and Equipment (2^nd ed.), Noyes Publications, Norwich (USA), 2007
4. Shuler M. L., Kargi F. Biochemical Engineering - Basic Concepts (2^nd ed.), Prentice Hall, New Jersey (USA), 2002