Biochemical engineering and bioprocess techniques

Course Coordinator

ECTS points:


Course number:

Course Description

The course containing three methodical units:

  1. Hydrodynamic characterization of bioreactors
  2. High structured mathematical models of metabolic flux and bottleneck detection
  3. Scale-up methods in bioprocess engineering

1) Hydrodynamic characterization of bioreactors:

Measuring of mixing time, circulation time, homogenization time, its distribution, methods for evaluation (delta function and step methods, conductivity method, pH-method, photometric methods,  radioactive tracers, radio transmitter method, temperature delta and step method, single and multi-sensors systems)

2) High structured mathematical models of metabolic flux and bottleneck detection

Structuring of mathematical models of metabolic pathways, metabolic modelling of primary metabolic products - direct related and partially related to growth, as well as the modelling of secondary metabolites synthesis). Modelling of prokaryote live-cycle, NMR and MS technics as a tool for model evaluation, sensitivity analysis and error analysis.

3) Scale-up methods in bioprocess engineering

Fundamental and semi-fundamental methods, dimensional analysis and regime analysis, rules of thumb method, try and error method.


Lectures: 20 hours

Seminars: 10 hours

Description of course requirements:

Preparation and defense of written seminar and oral exam


  1. Duran P. M. Bioprocess Engineering Principles (2nd ed.), Academic Press, Oxford (UK), 2012
  2. Marić V., Šantek B., Biokemijsko Inženjerstvo, Golden Marketing-Tehnička knjiga, Zagreb (HR), 2009
  3. Vogel H.C., Todaro, L.C. Fermentation and Biochemical Engineering Handbook: Principles, Process Design and Equipment (2nd ed.), Noyes Publications, Norwich (USA), 2007
  4. Shuler M. L., Kargi F. Biochemical Engineering - Basic Concepts (2nd ed.), Prentice Hall, New Jersey (USA), 2002