Isolation and Purification of Biotechnology Products

Course Description

COURSE CONTENT

1. Processes for separation of suspended particles from cultivation media

• L: Processes for separation of suspended particles from cultivation media: sedimentation, coagulation, flocculation and flotation (4 h)
• S: Calculation of the process for separation of suspended particles (4 h)

2. Techniques for microbial cells disruption

• L: Techniques for microbial cells disruption (2 h)
• S: Calculation of different techniques for microbial cells disruption (2 h)

3. Two phase extraction processes

• L: Two phase extraction processes
• S: Calculation of the two phase extraction process (4 h)
• P: Liquid – solid extraction process (5 h)

4. Adsorption

• L: Adsorption (2 h)
• S: Calculation of the adsorption process in columns with fixed and mixed adsorbent layer (2 h)

5. Distillation

• L: Distillation (2 h)
• S: Calculation of the distillation process (2 h)
• P: Distillation of multi-components liquid mixtures (5 h)

6. Membrane techniques for separation of biotechnological products

• L: Membrane techniques for separation of biotechnological products (4 h)
• S: Calculation of micro-, ultra- and nano-filtration processes as well as reverse osmosis (4 h)

7. Membrane techniques for gas and low volatile compounds separation

• L: Membrane techniques for gas and low volatile compounds separation (2 h)
• S: Calculation of membrane systems for separation of gasses and low volatile compounds (2 h)

8. Chromatography in industrial scale

• L: Chromatography in industrial scale (2 h)
• S: Calculation of industrial chromatographic systems (2 h)

9. Electro-dialysis

• L: Electro-dialysis process (2 h)
• S: Calculation of electro-dialysis processes (2 h)

10. Drying as a final phase of biotechnological products purification

• L: Drying as a final stage of separation process – crystals, biopolymers, proteins and microbial biomass (2 h)
• S: Calculation of crystals, biopolymers, proteins and microbial biomass drying process (4 h)
• P: Drying process of crystals, biopolymers, proteins and microbial biomass (5 h)

11. Crystallization as a final phase of biotechnological products purification

• L: Crystallization process and types of crystallizers (2 h)
• S: Calculation of crystallizers with heating or cooling systems (2 h)

12. Validation and registration procedures in pharmaceutical industry

• L: Validation and registration procedures in pharmaceutical industry (2 h)

LEARNING OUTCOMES

• calculate parameters for separation of suspended particles (sedimentation, flocculation and flotation) from cultivation media
• establish and manage flocculation and flotation process of different biotechnological products
• propose and manage adequate technique for microbial cells disruption
• establish and manage the extraction process of different biotechnological products
• establish and manage the adsorption and chromatographic processes of different biotechnological products
• establish and manage the distillation process of different low volatile biotechnological products
• establish and manage the crystallization and drying processes of different biotechnological products
• establish and manage the different membrane processes for separation of biotechnological products
• establish and manage the electrophoresis and electro-dialysis processes of different biotechnological products
• manage the validation and registration procedures for different biotechnological products