Molecular-genetic methods in control of microbiological food safety

Course Description

COURSE CONTENT

Fields of application of molecular genetic methods. The structure, and function of DNA molecule. Genes and genomes of prokaryotes and eukaryotes. The purpose and objectives of the molecular genetic analysis. DNA sequences suitable for analysis and principles of laboratory procedures of genotyping. PCR, qPCR, RAPD, DNA electrophoresis, cleavage of DNA with restriction enzymes, RFLP, Southern blotting, DNA sequencing, FISH, microarray, AFLP. Presentation and discussion of scientific papers in which molecular-genetics methods were applied.

LEARNING OUTCOMES

1. Create a workflow of molecular genetic methods such as PCR, qPCR, RAPD, RFLP, Southern blot, DNA sequencing, AFLP, microarray and analysis of tandem and dispersed repetitive DNA as well as explain these methods at the molecular level.
2. Predict and interpret results of molecular genetic analysis.
3. Propose the appropriate method for a specific analysis and propose a modification of the existing method.
4. Analyze and evaluate scientific paper in which molecular genetic methods are employed and present it to colleagues, answer questions from the audience and participate in scientific discussions.

Lectures

20 (10 lectures + 10 seminars)

Literature

1. Dale, J. W. and Park S. F. (2004), Molecular Genetics of Bacteria, J. Wiley & Sons
2. Lewin, B. (2008), Genes IX, Pearson Education
3. Brenner, S. and J.K. Miller (eds.) (2001), Encyclopedia of Genetics, Academic Press
4. Principles of gene manipulation and genomics, 7th edition, S.B. Primrose and R.M. Twyman, Blackwell Publishing, 2006.
5. Gene cloning and DNA analysis, 5th edition, T.A. Brown, Blackwell Publishing, 2006.
6. Molecular cloning: A laboratory manual, 3rd ed. Vols 1, 2 and 3. J. Sambrook, E.F. Fritsch and T.Maniatis, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 2001.
7. Short protocols in molecular biology, 5th ed. Vols 1 and 2. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, J. Willey and Sons, 2002.
8. Original scientific papers