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Culture of animal cells is widely used for production of viral vaccines, monoclonal antibodies and various therapeutic proteins. In order to obtain products of consistent quality, a development of well controlled production process is required. Using stable cell lines and optimal composition of culture media play a key role in the process. Defining and maintaining conditions during all steps of production and product formulation are to ensure the desired product quality and quantity. Research of stem cells for regeneration of damaged tissue and organ formation is another important field of mammalian cells application.
Through the practical work of this module, students will learn how to use basic laboratory equipment for the establishment and cultivation of animal as well as to monitor the main growth and metabolism parameters of animal cells in culture.
LEARNING OUTCOMES
Lectures: 10 h
Practical work: 15 h
Examination: Seminar and oral exam
Freshney, RI: Culture of Animal Cells – a manual of basic technique, fourth edition, Wiley-Liss Inc., New York, 2000.
Ozturk SS, Hu W-S: Cell Culture Technology for Pharmaceutical and Cell-Based Therapies. Taylor & Francis Group, New York, London, 2006
Castilho LR, Moraes AM, Augusto EFP, Butler M: Animal Cell Technology: From Biopharmaceuticals to Gene Therapy, Taylor & Francis Group, New York, London, 2008
Hauser H, Wagner R: Animal Cell Biotechnology in Biologics Production. Walter De Gruyter GmbH, Berlin, Munchen, 2015