HPLC-analysis of Low Molecular Weight Compounds

Course Description

COURSE CONTENT

This course is designed through three methodological units: (1) Basic principles of HPLC where students will consider principles of separation and mechanisms of HPLC and types of HPLC; (2) Performing chromatographic analysis where students will be introduce form practical approaches to all the steps in creating HPLC methods including sample preparation, selection and implementation of instrumental analysis and validation of the method; (3) Solving problems in the chromatographic analysis where student will be introduce how to recognize and resolve basics problems during HPLC analysis (technical and analytical).

LEARNING OUTCOMES

• define basic principles of separation and mechanisms of HPLC and types of HPLC
• select suitable method for sample preparation and to use gained knowledge to design HPLC analysis
• recognize and resolve basics problems during HPLC analysis (technical and analytical)
• consider and choose the correct method of analytical signals processing and to interpret the results of the analysis

Literature

1. I. Radojčić Redovniković: HPLC niskomolekulskih spojeva, (internal script)
2. HPLC Troubleshooting Guide, Phenomenex, Inc. USA., 2008.