Technology of Vitamin and Hormone Production

ECTS points:
4

Program:
preddiplomski

Course number:
39785 (PDBT) i 53713 (DBPI)

Course Description

COURSE CONTENT

  • Vitamins - definition, classification, nomenclature, chemical composition and role in the organism.
  • Water soluble vitamins-B-vitamins, vitamin C. Structure, properties  and industrial production.
  • Fat soluble vitamins- vitamins A,D, E i K. Stucture, properties and industrial production
  • Mass balance, quantification and yield of vitamine sinthesys process (from raw material to product)
  • Hormones - definition, classification, nomenclature, chemical composition and role in the organism
  • Steroid hormones- androgens, estrogens, progestogens and corticosteroids. Raw materials and production. Derivates of steroid hormones and application as anabolics.
  • Polipeptide hormones. Insulin. Insulin production by E. coli i S. cerevisiae. Growth hormone and production-isolation from natural sources and rDNA process by E. coli.
  • Erythropoietin-production. Gonadotropic hormones and production. Purification processes of rDNA hormones.
  • Plant hormones-role, chemical composition and role in plants.
  • Application of anabolics in humans and animals-specific examples
  • Production of GM plants with increase vitamine content
  • r Growth hormone in milk production

LEARNING OUTCOMES

  • define the role of vitamins and hormones in organism
  • compare chemical and biotechnological processes of vitamine production
  • explain and compare processes of steroid and peptide hormones
  • discuss new areas of vitamines and hormones application (rDNA technology)
  • calculate the mass balance and yield during the synthesis, isolation and quantitative determination of vitamins and hormones