Course Coordinator |
ECTS points: |
Program: |
Course number: |
COURSE CONTENT
Lectures: Control of gene expression: principles of transcriptional regulation in prokaryotic and eukaryotic cells; structure and function of RNA polymerase, general and specific transcription factors and coactivator protein complexes; chromatin modifying complexes, and mechanism of chromatin remodelling; processing of pre-mRNA. Biochemical methods and in vivo methods in study of transcriptional regulation. Splicing mechanism and alternative splicing, posttranscriptional regulation of gene expression. Postsynthetic modification of proteins. Mechanisms of protein recognition and targeting to cellular compartments; protein secretion; protein glycosylation.
Laboratory Practice: Yeast transformation method and examining the mechanism of transcription regulation of the yeast PHO5 gene by using regulatory mutants.
LEARNING OUTCOMES