Molecular Biology

Course Coordinator

ECTS points:
4

Program:
diplomski

Course number:
53243

Course Description

COURSE CONTENT

Lectures: Control of gene expression: principles of transcriptional regulation in prokaryotic and eukaryotic cells; structure and function of RNA polymerase, general and specific transcription factors and coactivator protein complexes; chromatin modifying complexes, and mechanism of chromatin remodelling; processing of pre-mRNA. Biochemical methods and in vivo methods in study of transcriptional regulation. Splicing mechanism and alternative splicing, posttranscriptional regulation of gene expression. Postsynthetic modification of proteins. Mechanisms of protein recognition and targeting to cellular compartments; protein secretion; protein glycosylation.

Laboratory Practice: Yeast transformation method and examining the mechanism of transcription regulation of the yeast PHO5 gene by using regulatory mutants.

LEARNING OUTCOMES

  • understand basic principles of gene expression process in prokaryotic and eukaryotic cells
  • describe, explain and compare the molecular mechanisms of transcriptional regulation of gene expression in prokaryotic and eukaryotic cells
  • describe and explain mechanisms of post-transcriptional regulation of gene expression in eukaryotic cells
  • describe principles and apply some of the modern biochemical and molecular biology methods used in investigation of transcriptional regulation
  • choose right biochemical methods in investigation of gene expression process
  • describe and explain the molecular mechanism of recognition and sorting of proteins in cellular organelles, secretion of proteins and the process of protein glycosylation