Renata Teparić, PhD

Biography

RESEARCH INTERESTS

• Study of biochemistry and molecular biology of the yeast
• Study of yeast cell wall proteins
• Investigation of interaction among cell wall constituents that may be responsible for the incorporation of proteins into the cell wall
• Surfice display of recombinant proteins in yeast

EDUCATION

2005., University of Zagreb Faculty of Food Technology and Biotechnology, Ph.D. in Molecular Biotechnology

Thesis: ˝ Saccharomyces cerevisiae cell wall proteins: binding to the cell wall and possible physiological functions˝

1999., University of Zagreb Faculty of Science, M.Sc. in Chemistry - Biochemistry
Thesis: ˝Influence of the cell wall proteins Bgl2p and Ccw12p on structure and properties of the Saccharomyces cerevisiae cell wall˝

1994., University of Zagreb Faculty of Food Technology and Biotechnology, B.Sc. in Biotechnology

WORK EXPERIENCE

 2019. – present University of Zagreb Faculty of Food Technology and Biotechnology, Zagreb, Croatia

• Full professor
• Courses: Biochemistry I; Biochemistry II; Protein Purification and Characterization; Biochemical Analytics, Biochemical Function of Vitamins and Ions in Food and Nutrition, Cell walls of industrial microorganisms, Nutritional biochemistry and biochemical changes in food
• Scientific project: Biotechnological application of surface engineering by incorporation of heterologous proteins in the yeast cell wall

 2013. - 2019. University of Zagreb Faculty of Food Technology and Biotechnology, Zagreb, Croatia

• Associate professor
• Courses: Biochemistry I; Biochemistry II; Protein Purification and Characterization; Biochemical Analytics, Biochemical Function of Vitamins and Ions in Food and Nutrition
• Scientific project: Molecular mechanisms for incorporation of homologous and heterologous proteins in the yeast cell wall and their application in biotechnology

 2008.-2013. University of Zagreb Faculty of Food Technology and Biotechnology, Zagreb, Croatia

• Assistant professor
• Courses: Biochemistry I; Biochemistry II; Protein Purification and Characterization; Biochemical Analytics

2005.-2008.University of Zagreb Faculty of Food Technology and Biotechnology, Zagreb, Croatia

• Postdoctoral Researcher and Teaching Assistant
• Courses: Biochemistry I; Biochemistry II; Protein Purification and Characterization; Biochemical Analytics

1999.- 2005. University of Zagreb Faculty of Food Technology and Biotechnology, Zagreb, Croatia

• PhD student and Teaching Assistant
• Courses: Biochemistry I; Biochemistry II; Protein Purification and Characterization; Biochemical Analytics

1994.- 1999. University of Zagreb Faculty of Food Technology and Biotechnology, Zagreb, Croatia

• MSc student and Teaching Assistant
• Courses: Biochemistry I; Biochemistry II; Protein Purification and Characterization

Professional memberships
FEBS society, FEMS society

PUBLICATIONS

1. R. Vuković and V. Mrša (1995) Structure of the Saccharomyces cerevisiae cell wall, Croatica Chem. Acta 68, 597-605. 

2. R. Teparić and V. Mrša (1997) Purification of yeast periplasmic proteins using protein   adsorption to glucan, Food Technol. Biotechnol. 35, 29-32.

3. V. Mrša, M. Ecker, C. Cappellaro, R. Teparić and W. Tanner (1999) Saccharomyces    cerevisiae cell  wall proteins, Food Technol. Biotechnol. 37, 21-27.  

4. R. Teparić, I. Stuparević and V. Mrša (2004) Increased mortality of Saccharomyces  cerevisiae  cell wall protein mutants, Microbiology 150, 3145-3150.  

5. R. Teparić, I. Stuparević and V. Mrša (2007) Binding assay for incorporation of alkali-extractable proteins in the Saccharomyces cerevisiae cell wall, Yeast 24, 259-266.

6. R. Teparić, I. Stuparević, V. Mrša. (2010) Incorporation of homologous and heterologous proteins in the Saccharomyces cerevisiae cell wall. Food technology and biotechnology 48, 317-328.

7. D. Đikić, I. Landeka, F. Knežević, A. Mojsović-Ćuić, V. Benković, A. Horvat-Knežević, G. Lončar, R.Teparić, D. Rogić (2011) Carbendazim Impends Hepatic Necrosis when combined with Imazalil or Cypermethrin. Basic Clin Pharmacol Toxicol. 110, 433-440.

8. R.Teparić, B. Didak, E. Ščulac, V. Mrša (2013) Genetic immobilization of RNase Rny1p at the Saccharomyces cerevisiae cell surface. The Journal of General and Applied Microbiology. 59, 75-82.

9. R. Teparić, V. Mrša (2013) Proteins involved in building, maintaining and remodeling of yeast cell walls. Current Genetics. 59, 171-185.

10. A. Bielen, R. Teparić, D.Vujaklija, V. Mrša (2014) Microbial anchoring systems for cell-surface display of lipolytic enzymes. Food Technol. Biotechnol. 52, 16–34. 

11. I. Čanak, A. Berkics, N. Bajcsi, M. Kovacs, A. Belak, R. Teparić, A. Maraz, V. Mrša (2015) Purification and Characterization of a Novel Cold-Active Lipase from the Yeast Candida zeylanoides. Journal of molecular microbiology and biotechnology 25, 403-411.

12. D. Borovikova, R. Teparić, V. Mrša, A. Rapoport (2016) Anhydrobiosis in yeast: cell wall mannoproteins are important for yeast Saccharomyces cerevisiae resistance to dehydration. Yeast 33, 347-353.

13. R.Teparić, V. Mrša (2016) Overview of systems and techniques for surface display of recombinant proteins in yeast S. cerevisiae. Applied food biotechnology 3, 3-14.

14. A. Grbavac, I. Čanak, I. Stuparević, R. Teparić, V. Mrša (2017) Proteolytic processing of the Saccharomyces cerevisiae cell wall protein Scw4 regulates its activity and influences its covalent binding to glucan, Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. 1864, 507–515

15. Sk A. Hossain, D. Švec, V. Mrša, R. Teparić (2018) Overview of Catalytic Properties of Fungal Xylose Reductases and Molecular Engineering Approaches for Improved Xylose Utilisation in Yeast. Applied food biotechnology 5, 47-58.  

16. Sk A. Hossain, R. Teparić, V. Mrša (2019) Comparison of two models of surface display of xylose reductase in the Saccharomyces cerevisiae cell wall. Enzyme and Microbial Technology 123, 8-14. 

17. M. Lozančić, A. Sk. Hossain, V. Mrša, R. Teparić (2019) Surface Display - An Alternative to Classic Enzyme Immobilization. Catalysts 9, 728. 

18. R. Teparić, M. Lozančić,V. Mrša (2020) Evolutionary Overview of Molecular Interactions and Enzymatic Activities in the Yeast Cell Walls. International Journal of  Mololecular Sciences 21, 8996.  

19. M. Lozančić, B. Žunar, D. Hrestak, K. Lopandić, R. Teparić, V. Mrša (2021) Systematic Comparison of Cell Wall-Related Proteins of Different Yeasts J. Fungi 7, 128. 

20. M. Hunić, R. Teparić, M. Kovačević, and L. Barišić (2022) The Chloroquine Story in the First Year of the COVID-19 Pandemic. Kem. Ind. 71, 473−484.

21. T. Martinić Cezar, M. Lozančić, A. Novačić, A. Matičević, D. Matijević, B. Vallée, V Mrša, R. Teparić, B. Žunar (2023) Streamlining N-terminally anchored yeast surface display via structural insights into S. cerevisiae Pir proteins Microbial Cell Factories 22, 174.

22.   T. Martinić Cezar, A. Paić, S. Prekpalaj, R. Teparić, B. Žunar, I. Stuparević (2024) Measuring the capacity of yeast for surface display of cell wall-anchored protein isoforms by using β-lactamase as a reporter enzyme. FEBS Open Bio.                                       

1.  R. Vuković, M. Hudina-Domladovec and V. Mrša (1994) Molecular organization of the Saccharomyces cerevisiae cell wall, Prehrambeno-tehnol. Biotehnol. Rev. 32, 99-102.

2.  I. Landeka, R. Teparić, T. Perica, D. Đikić, D. Rogić (2010) Effects of Dietary Lipids on Lipoprotein Profile, Croatian Journal of Food Technology, Biotechnology and Nutrition 5, 114-126.

3. I. Landeka, D. Đikić, I. Radišić, R. Teparić, V. Bačun-Družina, D. Rogić (2011) The Effects of Olive and Pumpkin Seed Oil on Serum Lipid Concentrations, Croatian Journal of Food Technology, Biotechnology and Nutrition 6, 63-68.

4. R. Teparić, I. Landeka, J. Tomić, D. Đikić (2012) Influence of Dietary Treatment on Lipid Metabolism in Metabolic Syndrome. Croatian Journal of Food Technology, Biotechnology and Nutrition 7, 69-77.    

5. R. Teparić, B. Didak, I. Landeka (2012) Overview of Saccharomyces cerevisiae Ribonucleases. Croatian Journal of Food Technology, Biotechnology and Nutrition 7, 141-151.