Priprava, analiza i primjena oligonukleotida

Suradnik

ECTS bodovi:
3

Studij:
diplomski

Opis predmeta

Sinteza oligonukleotida korištenjem automatiziranih sintetizera na čvrstom nosaču postala je svakodnevna praksa u kemijskim, biokemijskim i biološkim istraživačkim laboratorijima. Oligonukleotidi sastavni su dio genetičkog materijala (DNA, RNA) svake stanice, te se kao takvi intenzivno rabe u laboratorijskim manipulacijama nukleinskim kiselinama (npr. PCR metode, sekvencioniranje, mutacijskim studijama, hibridizacijskim studijama, RFLP-analizama bioloških populacija, antisens terapijske svrhe, …). Sintetski oligonukleotidi danas se koriste u svakodnevnim istraživanjima u DNA/RNA kemiji, biokemiji, biotehnologiji, molekularnoj biologiji, genetici, medicini, farmaceutskim istraživanjima, forenzici, DNA-dijagnostici, DNA-čipovima – genomika.

Nastava

Vrste nastave		sati
oznaka	naziv	sati
P	Predavanja	15
A	Auditorne vježbe
L	Laboratorijske vježbe	12
PK	Vježbe u praktikumu
S	Seminar	12
T	Terenske vježbe

Uvjeti za dobivanje potpisa

Predavanja, seminar, vježbe

Način polaganja ispita:

Pismeno

Usmeno

Predavanja [2]

Naziv datoteke	Veličina	Ubačeno/mijenjano
ANALIZA I IZOLACIJA SINTETSKIH OLIGONUKLEOTIDA	1,58 MB	02.09.2022. 17:05
PRIPRAVA, ANALIZA I PRIMJENA OLIGONUKLEOTIDA	727,04 kB	02.09.2022. 17:05

Obavijesti [0]

Naziv datoteke	Veličina	Ubačeno/mijenjano

Literatura

Obvezna literatura

red. broj	Naziv
1.	S.L. Becaucage, R.P. Iyer, Synthesis of Oligonucleotides in Tetrahedron Report Number 309, Tetrahedron, 53 (1997) 2223-2311. ; Functionalization of Oligonucleotides in Tetrahedron Report Number 329, Tetrahedron, 49 (1993) 1925-1963.; The Synthesis of Modified Oligonucleotides in Tetrahedron Report Number 335, Tetrahedron, 49 (1993) 6123-6194.
2.	Protocols for Oligonucleotides and Analogs: Synthesis and Properties; Methods in Molecular Biology (S. Agrawal, Ed.) Humana Press Inc., Totowa, NJ, USA (1993); Protocols for Oligonucleotide Conjugates: Synthesis and Analytical Techniques; Methods in Molecular Biology (S. Agrawal, Ed.) Humana Press Inc., Totowa, NJ, USA (1994).
3.	Chemistry of Nucleosides and Nucleotides, Vol. 1, (L.B. Townsend, Ed.) Plenum Press, New York, NY, USA (1989).
4.	Synthesis and Applications of DNA and RNA (S.A. Narang, Ed.) Academic Press, Inc., Orlando, FL, USA (1987).
5.	DNA Microarrays: A Practical Approach, (M. Schena, Ed.) in The Practical Approach Series (B.D. Hames, Seried Ed.), Oxford University Press, Oxford, UK (2000); Evaluating and Isolating Synthetic Oligonucleotides: The Complete Guide, Applied Biosystems.

Preporučena literatura

red. broj	Naziv
1.	S.L. Becaucage, R.P. Iyer, Synthesis of Oligonucleotides in Tetrahedron Report Number 309, Tetrahedron, 53 (1997) 2223-2311. ; Functionalization of Oligonucleotides in Tetrahedron Report Number 329, Tetrahedron, 49 (1993) 1925-1963.; The Synthesis of Modified Oligonucleotides in Tetrahedron Report Number 335, Tetrahedron, 49 (1993) 6123-6194.
2.	Chemistry of Nucleosides and Nucleotides, Vol. 1, (L.B. Townsend, Ed.) Plenum Press, New York, NY, USA (1989).
3.	Synthesis and Applications of DNA and RNA (S.A. Narang, Ed.) Academic Press, Inc., Orlando, FL, USA (1987).
4.	Evaluating and Isolating Synthetic Oligonucleotides: The Complete Guide, Applied Biosystems. DNA Microarrays: A Practical Approach, (M. Schena, Ed.) in The Practical Approach Series (B.D. Hames, Seried Ed.), Oxford University Press, Oxford, UK (2000)
5.	Protocols for Oligonucleotides and Analogs: Synthesis and Properties; Methods in Molecular Biology (S. Agrawal, Ed.) Humana Press Inc., Totowa, NJ, USA (1993) Protocols for Oligonucleotide Conjugates: Synthesis and Analytical Techniques; Methods in Molecular Biology (S. Agrawal, Ed.) Humana Press Inc., Totowa, NJ, USA (1994).