Protein Engineering

Course Description

COURSE CONTENT

Lectures:

1. Overexpression and purification of recombinant proteins (3 h)
2. Methods of protein engineering to obtain modified and/or improved proteins (6 h)
3. Protein interactions (3 h)
4. Protein Engineering with non-natural amino acids (3 h)
5. pplication of protein engineering in biotechnology, food technology and medicine (6 h)

Seminar:

1. Seminar presentation of recent relevant paper by students (presentation  + discussion) (3 h)

LEARNING OUTCOMES

• to propose and explain procedures that allow over-expression and recombination of recombinant proteins
• apply methods of protein engineering to obtain modified and / or improved proteins
• analyze and propose methods for monitoring protein interactions
• explain the basic principles of the methodology for the installation of non-natural amino acids in proteins
• explain the application of protein engineering in biotechnology, food technology and medicine
• apply the acquired knowledge in the field of microbiology, microbial physiology, molecular biology, genetics and bioinformatics for the purpose of producing biotechnological products
• interpret, discuss and explain original scientific work in the form of oral communication