1. The role of intestinal microflora in the intestinal tract
L: Human microbiome. Composition of intestinal microflora (microbiota). Historical overview of the impact of intestinal microflora in the host health. Modern systems used for the research of intestinal microflora composition, its metabolic activity and its role in the organism. The share of lactic acid bacteria (Lactobacillus and Bifidobacterium) in the autochthonous intestinal microflora and their impact on health.
S: Phenotypic and genotypic identification and characterisation of bacteria in the intestinal tract. Metabolic activity of intestinal microflora in the intestinal tract.
L: Sources of nutrients for the microbial population of the colon. The main products of bacterial metabolism in the intestinal tract. The role of short chain fatty acids (SCFA) in the intestinal tract. Molecular mechanisms of adaptation of lactic acid bacteria to stress conditions in the gastrointestinal tract.
E: Research of survival of lactic acid bacteria in the simulated conditions of the gastrointestinal tract.
2. Influence of nutrition on intestinal microflora
L: Influence of nutrition on composition and metabolic activity of colon microflora. Influence of probiotics and prebiotic substrates on intestinal microflora. Inulin type fructan, galactooligosaccharides and xylooligosaccharides – production and application. Bifidogenic and butyrogenic effects.
E: Determination of autoaggregation and coaggregation properties of selected probiotic bacteria.
3. Influence of intestinal microflora balance disorder on health
L: Causes of intestinal microflora balance disorders. Connection of intestinal microflora composition with the occurrence of metabolic disorders and diseases. Nutritional and microbial modulation of carcinogenesis in the intestinal tract. Models of microbial cells adhesion to intestinal epithelial cells and competitve exclusion of pathogenic bacteria. Concept of pharmaco-metabolomics. Intestinal microflora as the target of a new therapeutic strategy.
S: Influence of intestinal microflora composition on the occurrence of metabolic disorders and diseases.
E: In vitro research of antimicrobial activity of lactic acid bacteria against selected species of Bacillus, Staphylococcus, Salmonella and Escherichia genus.