Laboratory for Cell Culture Technology and Biotransformations


Igor Slivac, PhD

Phone: + 385 1 4605 278

 The Laboratory was founded in 1960 within the Department of Organic Chemistry and Technology of Food and Technology Department at the Faculty of Technology, University of Zagreb under the name of Laboratory for Chemistry and Technology of Vitamins and Hormones. Since 1980 it has been a part of the Department of Biochemical Engineering of the Faculty of Food Technology and Biotechnology, University of Zagreb. Expanding the area of its activity, in 1996 the Laboratory was renamed as the Laboratory for Cell Technology, Application and Biotransformation.

At the beginning of the establishment, scientific activity within the Laboratory was in the field of synthesis of intermediates and derivatives of various vitamins. Since 1987, scientific research has expanded to the field of synthesis and metabolic mechanisms of steroid and pituitary hormones, biological activity of natural plant secondary metabolites (glucosinolates and polyphenols), biocatalysis in obtaining enantiomeric pure compounds, studying of natural heterocyclic compounds and the influence of xenobiotics, especially pesticides on reproductive functions. Particularly important aspect of Laboratory scientific activity was equipping the laboratory for animal cell culture, which started researches related to the development and application of animal technology and vaccine production in bioreactor systems. Research has also been focused on the use of in vitro cell systems for monitoring of environmentally presented substances.

Current Scientific expertise

The research interest of the Laboratory members currently covers two areas:

  1. Cell culture media development for improved animal cell growth and productivity in technology of recombinant protein production. The cell growth media is supplemented with compounds from processed natural substances and tested in cell culture for its beneficial properties. Current focus is given to the protein hydrolysates of plant origin that are tested as partial substituent of proteins contained in animal serum. The same compounds are assessed for their biological activity. Furthermore, we study in vitro cytotoxicity of different natural and synthetic compounds.
  2. Our research team in collaboration with several institutions is dedicated to preparation, characterization and implementation of NADES in food technology, biotechnology and chemical technology. In that time we published more than 20 scientific paper on this subject in highly-impacted journals. Gained knowledge and experience in this field encourage us to advise and offer NADES service, which provides all in one preparation and characterization of NADES together with support in developing of eco-friendly technologies using NADES.We offer our customers the opportunity to jointly develop completely new products and bring them to market, using our NADES and profound know-how.


Name and surname Academic title Job title
Višnja Gaurina Srček PhD Full Professor
Zlatko Kniewald PhD Full Professor
Ivana Radojčić Redovniković PhD Full Professor
Igor Slivac PhD Associate Professor
Kristina Radošević PhD Assistant Professor
Marina Cvjetko Bubalo PhD Senior Scientific Assistant
Manuela Panić PhD Scientific Assistant
Marijan Logarušić mag. ing. Scientific Assistant
Mia Radović mag. ing. Scientific Assistant

Courses (preddiplomski)

Course Title Course number ECTS
HPLC-analysis of Low Molecular Weight Compounds 39865 3
Technology of Vitamin and Hormone Production 39785 (PDBT) i 53713 (DBPI) 4

Courses (diplomski)

Course Title Course number ECTS
Animal and plant cell technology 53245 (DMB) i 53226 (DBPI) 4
Basics of Tissue Engineering 66748 2
Biotransformations 53268 3
Interaction of Molecules and Receptors 53260 2
Phytoremediation 66750 3

Courses (doktorski)

Course Title Course number ECTS
Plant biotechnology 165646 3
Scientific projects and intellectual property protection 165648 3
Signal transduction in cells 165610 3
Stereoselective biotransformations 165609 3
Technology and application of animal cells 165635 3